What do you know? We have a winner. While the first debate was all about Obamacare, the second debate was all about tax reform. Most voters care little about Obamacare or the future of the ACA, but they do care a lot about taxes.
It is no secret to anyone who has been paying attention that Donald Trump is not a fan of free trade. Ever since he ran for president, he has been critical of free trade deals negotiated by the previous administration. We haven’t had a trade agreement in years. Trump campaigned on a promise to renegotiate NAFTA. If he is elected president, this is the deal that is likely to be renegotiated: www.nytimes.com
When your friends, colleagues and family tell you that you’re crazy, you don’t have to listen. But when people who are experts on the Constitution tell you that you are crazy, you might need to listen.Proliferation, migration, and viability of preadipocytes differentiated from human adipose tissue explants.
The aim of this study was to establish and characterize adipocyte-like cells differentiated from human adipose tissue. The tissue samples were enzymatically digested and the cell fraction was isolated and cultured for 7 d. At confluency, culture medium was replaced and the cells were induced to differentiate for 14 d. Two distinct phenotypes of cells were observed in the culture. The first phenotype was non-adipose-like, with a large nucleus and scant cytoplasm. The second phenotype was adipose-like, with small, rounded cells with multiple lipid droplets. The preadipocytes demonstrated expression of PPAR-gamma, C/EBP-alpha, and GAPDH and were not positive for CK19, CK18, ALDEF, PDGFRalpha, CD31, CD34, VE-cadherin, and mesenchymal markers. The cells migrated and proliferated, and maintained viability over a 7-d period. The cells expressed adipogenesis-related genes and proteins throughout the 14-d period. Preadipocytes of human adipose tissue obtained from lipoaspirate showed differentiation potential and maintained viability. Because they were positive for adipogenesis-related proteins, these preadipocytes could be useful tools for studying human fat metabolism.
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