Among the most common causes of inadequate blood collection samples are failure to find an appropriate vein, failure to aim correctly the needle at the vein, or failure to perform the blood collection in an ideal puncture-free area . The precordial area on the left or right side of the sternum is often targeted. When the needle is placed in a subcutaneous region, the needle goes entirely through the vein, but punctures the skin without producing any blood. This kind of puncture is called dry tap.
The patients' experiences in blood collection were significantly affected by professionals' attitudes, behaviors and attitudes during the procedures . Although the veins are close to the skin surface, vascular access was the most common cause of sample failure. This may be a limitation of the process of blood collection, but it also raises questions about the representativeness of models. Consequently, this may lead to inaccuracy in the conducting of experimental work and unusable blood samples, which can reduce the availability of quality samples to a considerable degree. In addition, venous puncture is the most common reason for the failure of blood sampling , .
Although it is important to have access to good research samples, this access can influence the study results and may affect the study performance. Secure storage and good preservation are key elements for maintaining the quality of the research samples. The preservation time of blood samples is related to the erythrocyte and leukocyte concentrations , which makes it compulsory to store the samples in good quality transportation containers with ice packs or dewars (Fig. 3.1). Once the samples are received, the samples should be immediately processed at 4°C , , otherwise the blood cells will begin to clot and the samples may lose their quality. It is important to use a transportation container with saturated vapor and that is protected from light (Fig. 3.2). Once the samples are taken, you can use the manufacturer's recommendations for storage and transportation of the blood samples. d2c66b5586