Food Handlers Exam 1004 Answers
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Early research on insect food webs used microscopy. This method has the advantage of being rapid, inexpensive, and sensitive. It is useful for tracking the movement of invertebrates from one food source to another, but has several limitations. First, it is not effective for assessing the content of insects, which may be eaten in large numbers or small quantities. The digestive tracts of insects, especially those of flying insects, have microfibrils that make visualization by light microscopy difficult. Structures that are not visible by conventional light microscopy are usually easily detected by scanning electron microscopy. Thus, scanning electron microscopy is often used to detect insect prey in the feces of predators (Calver, 1984; Jordan & Baker, 2000). The other major limitation of this method is that it provides only indirect evidence for ingestions. When insects are eaten and digested, the microfibrils break down and the insect is no longer recognizable as a morphological distinct structure (Jordan & Baker, 2000).
The most widely used serological methods for invertebrate food studies are the enzyme-linked immunosorbent assay (ELISA) and the enzyme immunoassay (EIA). Enzymes are used to catalyze chemical reactions, amplifying reaction rates by a million-fold or more. The reaction of an antibody with a specific antigen causes a permanent change to the antibody, which makes it detectable by other means (for example, by fluorescence). The detection of enzymatic reactions has become routine in molecular biology. The ELISA and EIA are used in dietary studies to detect the presence of antigenic proteins in an animal's tissue or feces.
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